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    How To Create An Antibody Panel For Flow Cytometry

    The flow cytometry (FC) is a tool for analyzing the physicochemical properties of the cells or components. To achieve good results, it is necessary to plan the tests properly, paying special attention to the design of the polychromatic panels and thus optimizing the sensitivity of the test. In this post we summarize the most critical steps on how to create an antibody panel for flow cytometry . 1.- Establish A Hypothesis After establishing a biological hypothesis, we should ask ourselves some questions that will allow us to properly plan the experiment: What population do you want to identify? What information do you want to extract from the data obtained? Are there commercial reagents available for…

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